Strains
Gluten-Degrading Probiotic: Lactic Acid Bacteria
Strain
Raw Material (Strain)
1. De-glu GLU70(GLU70)
2. De-glu MB0601(MB0601)
3. 5-Strain Probiotic Complex (ML-004, ML-036, ML-019, ML- 035, ML-117)
4. MF01
Function
Function
1. Gluten-degrading activity; Preventing, improving, or treating gliadin-induced inflammatory bowel disease (Patented)
2. Gluten-degrading activity (Patented)
3. Antibacterial and antiviral effect
4. Production of digestive enzymes
De-glu GLU70
A composition for preventing, improving, or treating gliadin-induced inflammatory bowel disease comprising of GLU70 as an active ingredient having gluten degradation activity
Experimental Groups
Test Substance
Dosage
Administered for 21 days
NC
DW
-
Gliadin
Gliadin
10mg / 250㎕ / mice
De-glu GLU70-L
Gliadin + De-glu GLU70 low concentration
40mg / kg / day
De-glu GLU70-H
Gliadin + De-glu GLU70 high concentration
400mg / kg / day
Normal intestinal
epithelial cell
Intestinal epithelial cells
damaged by gliadin
Degeneration of damaged intestinal epithelial cells inhibited by De-glu GLU70
Confirmed inhibition of gluten(gliadin)-induced degeneration of intestinal epithelial cells by GLU70
Experimental Groups
Test Substance
Dosage
NC
DW
-
Gliadin
Gliadin
10mg / 250㎕ / mice
PC
Gliadin + Vancomycin
10mg / kg / day
De-glu GLU70-L
Gliadin + De-glu GLU70 low concentration
40mg / kg / day
De-glu GLU70-H
Gliadin + De-glu GLU70 high concentration
400mg / kg / day
Decreased secretion of TNF-α (inflammatory cytokine)
Increased secretion of IL-10
(anti-inflammatory cytokine)
Decreased expression levels of inflammatory
proteins in De-glu GLU70-ingested groups
Reduced secretion of inflammatory cytokines, increased secretion of anti-inflammatory cytokines, and decreased expression of inflammatory proteins when feeding De-glu GLU70 strain with feed containing gliadin
De-glu MB0601
MB-0601 strain having gluten degradation activity and uses thereof
Traditional Fermented Food
Electron microscope image of De-glu MB0601
Isolation/Securing of Gluten-Degrading Lactic Acid Bacteria
De-glu MB0601
Add
Knead
Rest
Gluten Developed
Gluten degraded
.png)
Evaluation of gluten degradability of De-glu MB0601 strain
Comparing the difference between regular dough vs dough with gluten-degrading strain
Experiment #1 Regular dough (Resting time: 2 hours)
.jpg)
.jpg)
.jpg)
.jpg)
.jpg)
Confirmed that the elasticity of the dough is decreased by gluten-degrading lactic acid bacteria
Mice were fed meals that were mixed with our gluten-degrading flour (fermented with De-glu MB0601) or ordinary flour for 8 weeks. The results showed that as the content of gluten-degrading flour increased:
​
​
-
Blood gastrin content of the mice increased by up to 60% - Gastrin (a hormone secreted by G cells of the gastric glands) promotes gastric juice secretion and aids digestion
-
Secretion of Pancreatic amylase increased by up to 80% - Amylase is an enzyme that breaks down carbohydrates in wheat flour
Blood gastrin levels according to feeding gluten-degraded flour
.png)
Pancreatic amylase levels according to feeding gluten-degraded flour
Experiment #2 Dough with gluten-degrading strain added (Resting time: 2 hours)
5-Strain Probiotic Complex
Antibacterial and Antiviral Test Report
Antibacterial Test
Antiviral Test
Certificate of Deposit
MF01
[Digestive-Enzyme Producing Strain] New strain of Aspergillus and solid phase fermentation process of a Arecaceae kernel cake using the same
Produces various enzymes that break down carbohydrates (starch) as well as proteins and promotes the secretion of amylase to facilitate the breakdown, absorption, and digestion of flour